In Vitro Characterization of chIFITMs of Aseel and Kadaknath Chicken Breeds against Newcastle Disease Virus Infection

Newcastle disease (ND) is highly contagious and usually causes severe illness that affects Aves all over the world, including domestic poultry. Depending on virus’s virulence, it can impact nervous, respiratory, digestive systems cause up to 100% mortality. The chIFITM genes are activated in response viral infection. current study was conducted quantify mRNA of vitro ND It also examined its ability inhibit virus replication chicken embryo fibroblast (CEF) cells Aseel Kadaknath breeds. Results from showed expression significantly upregulated throughout period infected CEF both breeds compared uninfected cells. In breed, elevated levels chIFITM3 gene dramatically reduced growth, load 60% lower than breed. level chIFITMs ranged 2.39 11.68 log2 folds higher control CEFs consistently (p < 0.01) CEFs. Similar this, theIFN-γ expresses strongly quickly peaks at 13.9 fold 48 hpi. Based these cellular experiments, breed exhibits potential for greater tolerance Aseel. However, gain a comprehensive understanding resistance mechanisms chickens, further research involving vivo investigations crucial.